Putative endolysin

Author: bifs

August undefined, 2024

WebMethods: We propose a computational method for the prediction of endolysin and autolysin. First, a data set containing 27 endolysins and 41 autolysins is built. Then the protein is … WebMar 22, 2012 · Computational predictions and analyses of HolSMP. The complete 36,126-bp sequence of phage SMP has 48 open reading frames (ORF). ORF42, designated LySMP, encodes the putative endolysin.ORF43, which is 429 bp long and upstream of the lySMP gene, is predicted to encode a putative holin protein, designated HolSMP (Figure …

A Putative Amidase Endolysin Encoded by Clostridium ... - PubMed

WebMar 23, 2024 · To enable functional and structural studies of the putative Enc34 endolysin, the coding sequence of the ORF39 protein with a cleavable N-terminal hexahistidine-tag was cloned and expressed in ... WebJan 31, 2024 · Putative endolysin 117 was the only successfully overexpressed endolysin, and it was renamed LyJH1892. LyJH1892 showed potent lytic activity against both methicillin-susceptible S. aureus and MRSA and showed broad lytic activity against coagulase-negative staphylococci. puk thema kunstenaar

Frontiers Combination Effect of Engineered Endolysin EC340 …

WebThe 723 putative endolysins ranged from 72 to 578 residues in length, using 24 different catalytic domains and 18 different cell wall-binding domains organized with 89 different … WebMar 1, 2024 · that a 36.1-kbp putative phage remnant region exists only on the genome of C. perfringens st13 and is absent from the genomes of the other sequenced C. perfringens strains (Figure S2). A gene designated psa (gene ID; CPE1138), encoding a putative endolysin, was detected at one end of this region; a putative holin gene is also present in … WebSep 1, 2024 · Identification and overexpression of recombinant endolysin LysSTG2. From the genome sequence of phage STG2, the predicted open reading frame for a putative endolysin gene encoding a protein (LysSTG2) consisting of 138 amino acids (expected molecular mass of 15.3 kDa) was identified. puk system

Expanding the Database of Signal-Anchor-Release Domain

Evaluation of factors influencing expression and extraction of ...

WebApr 12, 2011 · We found that an endolysin gene (psm) in phiSM101, an episomal phage of enterotoxigenic C. perfringens type A strain, SM101 (Myers et al. 2006), encodes a … WebApr 10, 2024 · As a result, any dynamic endolysin-based infection power merchants must combine outer-membrane-permeabilizing marketers after remaining ... Wang G et al (2024) Biological properties and genomics analysis of vB_KpnS_GH-K3, a Klebsiella phage with a putative depolymerase-like protein. Virus Genes 55:696–706. Article CAS PubMed ... puk torneiWebFeb 15, 2024 · The gene (GenBank accession no. OL415943) encoding putative endolysin from bacteriophage PBEC131 was cloned into pET21a+ (Novagen, United States) using … puk vinaphone

"WebDec 9, 2024 · Here we introduce a putative phage lysin that specifically lyses vegetative C. botulinum Group I cells. This lysin, called CBO1751, efficiently kills cells of C. botulinum Group I strains at the ... " - Putative endolysin

Putative endolysin

WebMay 25, 2024 · Bacteriophage encoded endolysin as an alternative antibiotics is a peptidoglycan degrading enzyme with a low rate of resistance. Here, the engineered endolysin was developed to defeat multiple drug-resistant (MDR) Acinetobacter baumannii. First, putative endolysin PA90 was predicted by genome analysis of isolated … WebNov 22, 2024 · In the present work, a putative endolysin (AbLys1) was identified and characterized. Ab Lys1 was evaluated for its antimicrobial activity against a clinically isolated A. baumannii strain. The work aimed at the discovery of new antimicrobial agents towards A. baumannii cells as an alternative non-antibiotic strategy to combat this …

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WebNov 12, 2024 · In this study, the potential role of the VL1_T00003720 (denominated as Vl3720) gene, encoding a putative endolysin-like protein, has been investigated in the … WebMar 15, 2024 · Bioinformatics analysis using BLAST with LysK endolysin gene sequence as query allowed for the identification of 25 putative endolysin encoding genes. Cg and …

WebThis study investigates 2,628 putative endolysin genes identified in 183,298 bacteriophage genomes. Previously, bioinformatic approaches resulted in a database of 66 SAR-endolysins. WebClostridium perfringens is an often-harmful intestinal bacterium that causes various diseases ranging from food poisoning to life-threatening fulminant disease. Potential treatments include phage-derived endolysins, a promising family of alternative antimicrobial agents. We surveyed the genome of the C. perfringens st13 strain and identified an …

WebAn open reading frame homologous to the genes encoding several cell-wall hydrolyzing enzymes was identified on the genome of actinophage μ1/6. This open reading frame encoding the putative endolysin was amplified by polymerase chain reaction and cloned into the expression vector pET-21a. This gene consisted of 1182 bp encoding a 393 amino … WebMar 1, 2024 · Clostridium perfringens is an often-harmful intestinal bacterium that causes various diseases ranging from food poisoning to life-threatening fulminant disease. …

WebMar 1, 2024 · A Putative Amidase Endolysin Encoded by Clostridium perfringens St13 Exhibits Specific Lytic Activity and Synergizes with the Muramidase Endolysin Psm ...

WebMar 22, 2012 · Computational predictions and analyses of HolSMP. The complete 36,126-bp sequence of phage SMP has 48 open reading frames (ORF). ORF42, designated LySMP, … puk viettelWebApr 11, 2024 · Aiming at utilizing a phage endolysin as a therapeutic alternative to antibiotics, we surveyed the genome and bacteriophage sequences of C. perfringens. A phiSM101 muramidase gene (psm) revealed by this study can be assumed to encode an N-acetylmuramidase, since the N-terminal catalytic domain deduced from the gene shows … puk vossenWebNov 13, 2013 · The putative endolysin gene of IME-EF1 was cloned and fused with His tag sequence into vector pColdI. Through the induction of 1 mM isopropyl beta-D-thiogalactoside (IPTG), the transformed E. coli M15 expressed 30 kDa soluble protein, and the band can be probed using anti-His Tag antibody . puk von sim karteWebPreviously, bioinformatic approaches resulted in a database of 66 SAR endolysins. This manuscript almost doubles the list with 53 additional SAR endolysin candidates. Forty … puk yesssWebClostridium perfringens is an often-harmful intestinal bacterium that causes various diseases ranging from food poisoning to life-threatening fulminant disease. Potential … puka albanien puka alevikWebOct 7, 2014 · Resistance rates are increasing among several problematic Gram-negative pathogens, a fact that has encouraged the development of new antimicrobial agents. This … puka 2