Webcds2 <-reduceDimension (cds2, max_components = 2) cds2 <-orderCells (cds2) plot_cell_trajectory (cds2, color_by = "Stage") We clearly get a very different trajectory with the negative binomial, no branching of the blastocyst stage. Test instead with using top dispersed genes for pseudotime ordering. WebNov 8, 2024 · orderCells() will then "walk" along the trajectory, and as it encounters additional cells, it will assign them increasingly large values of Pseudotime. Value an …
monocle2 ordercells()函数报错-编程语言-CSDN问答
WebMar 2, 2024 · 师弟在做单细胞拟时序分析的时候遇到了报错: WebSearch all packages and functions. monocle (version 1.6.2). Description Usage Arguments graphe potentiel tâche
Monocle2报错的终极解决方案 - 知乎 - 知乎专栏
WebFeb 7, 2024 · Build site. In this vignette, we will process fastq files of the 10x 10k neurons from an E18 mouse with the kallisto bustools workflow, and perform pseudotime analysis with Monocle 2 on the neuronal cell types. Monocle 2 is deprecated, but it can be easily installed from Bioconductor and still has a user base. http://cole-trapnell-lab.github.io/monocle-release/docs/ WebAug 18, 2024 · 解决monocle2的orderCells报错的两种方法 by Biomamba 生信基地 此前我们给大家做了一系列monocle的教程用于帮助大家完成 拟时序分析 ,虽然是 边做边骂 monocle语法差、bug多、版本不兼容,但是monocle从 计算效率和效果 上来看仍然是市面上最优秀的拟时序分析软件之一。 graph equation for me